FastQCFastQC Report
Mon 27 Feb 2023
SRR3128752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066799
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129590.627008238343448No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC36420.17621452303779903No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35910.1737469391072862No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG34100.1649894353538975No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG28100.13595903617139354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61300.080.986951
AGGGATG37150.075.143865
ACGGGTA2550.073.721854
AGGGTAC9450.073.105735
ATAGGGC13150.072.9089363
TAGAGGG40950.072.533652
AGATAGG11200.072.265921
TAGGGTC5000.071.436474
TAGGGCG4150.071.345874
GAATAGG13650.071.016241
AGTAGGG34950.070.46292
TAAGAGG15650.069.1570741
ATAGAGG16000.069.1147841
TAGACGG3300.068.4463651
ATAGGGA20500.068.318583
TAGGGCA12500.068.0526354
AAGAGGG79300.067.977722
GAGGGAT29000.067.741484
TAAGGGA17500.067.1395343
GATAGGG30250.066.806612
Produced by FastQC (version 0.11.5)