FastQCFastQC Report
Mon 27 Feb 2023
SRR3128751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097314
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138550.6606068523835725No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC35760.170503796761RNA PCR Primer, Index 41 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG33480.15963274931650673RNA PCR Primer, Index 41 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG27570.1314538500195965Illumina PCR Primer Index 11 (95% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25740.12272840404441109No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61900.079.217311
AGGGATG36950.074.524715
CGTAGGG6300.073.394982
TAGGGTA4900.073.180994
AGTACGG1900.072.034341
TAGGGCA12550.071.80764
GTAGGGC10950.071.527793
AGTAAGG12900.071.341151
ATAGGGC12600.071.148193
TAGAGGG35200.070.5056462
AGGGAAT22600.070.486895
AGTAGGG34900.070.300552
ATAGGGA19050.069.597263
AAGAGGG76450.069.554822
AGGGTAC7900.068.999835
CGAAGGG22650.068.534382
AGAGGGC30150.068.0742343
TATAGGG15950.067.741622
TAGGGCG4200.067.403554
ATAAGGG30800.067.0971762
Produced by FastQC (version 0.11.5)