FastQCFastQC Report
Mon 27 Feb 2023
SRR3128750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165821
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134850.6226276317387264No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC37760.17434497126032114RNA PCR Primer, Index 41 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG36350.1678347379584924RNA PCR Primer, Index 41 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG29870.1379153678905136Illumina PCR Primer Index 11 (95% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25390.11723037130030597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62850.078.634221
AGTACGG2750.077.0206761
AGGGATG40250.076.7140355
GTACGGG6050.073.061492
CGTAGGG5550.072.865432
GAATAGG14000.070.93851
AGGGTAC9550.070.865445
TAGGGTC5950.070.298884
ATAGGGA18200.070.238123
GAGGGTA10200.070.035584
AAGGGCG11000.069.6418464
TAGAGGG40350.069.4574362
TAAGAGG17250.069.3062061
AAGAGGG76550.069.29152
CGAAGGG22500.068.967972
TAGGGCA10950.068.672244
GTAGGGC10750.068.6383063
AGATAGG13300.067.948071
AGGGCGA12750.067.8239445
AGTAGGG35850.067.81372
Produced by FastQC (version 0.11.5)