FastQCFastQC Report
Mon 27 Feb 2023
SRR3128749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282696
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140110.6137917620217497No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC39460.17286576924829236RNA PCR Primer, Index 41 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG37900.16603174491916575RNA PCR Primer, Index 41 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG30620.13413963138324156Illumina PCR Primer Index 11 (95% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27230.11928877082187028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66250.080.7935941
AGTACGG2900.076.229031
CGTAGGG5450.075.027372
AGGGATG38300.073.625865
TAGAGGG41350.072.2900542
AGGGAAT22050.072.255195
TAGGGCG4750.072.233014
GTACGGG5900.071.694772
ATAGGGC13350.071.1175163
AAGAGGG82050.070.571462
AAGGGCG12950.070.410664
TAAGAGG17050.070.3455051
ATAGAGG14300.070.059031
AGTAAGG14850.069.9981161
GTAGGGC10350.069.9337543
GGAGCTA51300.069.534829
AGGGTAC8400.069.377765
CGAAGGG24550.069.3033142
TAGGGCA10800.068.325434
TAAGGGT8300.067.9531563
Produced by FastQC (version 0.11.5)