FastQCFastQC Report
Mon 27 Feb 2023
SRR3128748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061349
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208621.012055697506827No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC27770.13471760483062306No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG24800.12030956427077609No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81150.083.171911
GAATAGG13700.075.396141
TAGGGTA5850.074.1569444
AGGGATG34900.074.0575265
TAGGGCA8100.072.768744
GGTAAGG12000.071.534531
GTAGGGT6900.070.389533
TAAGGGA17350.070.1197053
ATAGGGC12450.070.0684053
ACGGGTA1750.070.057594
AGTAGGG37300.070.035912
CGTAGGG6100.069.5716862
TAGAGGG38000.068.8698652
AGGGTAT10200.068.646445
AGGGTAC9650.068.1761865
ATAGGGA16400.068.143493
TAGGGAT14450.067.875864
GAGGGAT25950.067.77844
AAGGGAT36100.067.661634
ATAGAGG15350.066.677131
Produced by FastQC (version 0.11.5)