FastQCFastQC Report
Mon 27 Feb 2023
SRR3128747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086145
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205510.9851184840938668No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC28960.13882064765392627No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG25350.1215160020036958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76150.081.388171
TAGGGCA8750.073.5842744
AGATAGG12050.072.248711
TAGGGTA6000.072.0624544
AGGGATG33550.071.861675
ATAGGGC11800.070.8941653
GAATAGG14500.070.751211
AGGGTAC10350.069.928285
AGTAGGG34750.069.4167252
GGTAAGG14100.068.753291
ATAGGGA17950.068.074023
AAGGGAT34850.067.832484
ATAGCGG3650.067.043371
GAGGGAT27050.066.890744
AAGGGTA13850.066.848084
AATAGGG36300.066.841262
TAAGGGA17150.066.5908663
AAGAGGG86200.066.3872762
TAGAGGG37800.066.303572
AGTTAGG7550.066.071
Produced by FastQC (version 0.11.5)