FastQCFastQC Report
Mon 27 Feb 2023
SRR3128745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734247
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132350.7631554213442492No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC65430.37728189813792384No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC40170.23162790536757455TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG34320.19789568613928699No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG32620.18809316089345982No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC18570.10707817283235894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATG49050.081.2427755
CGTTTTT47900.079.5972441
AGGGTAC10850.078.392915
AGGGAAT20400.078.320715
AAGGGAT37850.077.378744
TAGGGTA6350.075.756934
AAGGGTA14700.075.0748444
GGATGGC41200.074.936967
GAGGGAT31650.074.804194
ACGGGTA1850.073.930254
GAATAGG8550.073.401971
GATGACC31850.072.591138
ATGGCAC38000.072.219959
GGGATGA36100.071.855476
CTACGAC12100.071.07125
TAGGGCA7700.070.4373554
TATAGGG12400.070.3632662
GGAATGC20150.069.963927
AGGGTAG8400.069.9292145
AGATAGG9950.069.7134251
Produced by FastQC (version 0.11.5)