FastQCFastQC Report
Mon 27 Feb 2023
SRR3128744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796662
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128790.7168293201503677No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC68240.37981545777669923No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC43280.24089116372472955TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG37040.20616009021173712No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG35990.20031591918791627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATG48600.082.392355
AGGGTAC11900.079.778895
AAGGGAT41150.078.920864
CGTTTTT48550.077.514571
GAGGGAT29650.077.194774
AAGGGTA15250.076.1220864
ACGGGTA2500.075.1975254
AGGGAAT22100.074.857275
TAGGGCG2400.074.4142154
GGGATGA38100.074.3833856
ATGGCAC38150.073.053969
GGATGGC42450.072.407517
GATGACC33050.072.097488
AGAGGGA43500.071.5240863
GGATGAC34600.071.176867
GGTAAGG9000.071.0851751
CTACGAC12900.070.679855
TAAGCGG4400.070.5624851
GGAATGC20800.070.497687
CGAGGGA12250.070.2099463
Produced by FastQC (version 0.11.5)