FastQCFastQC Report
Mon 27 Feb 2023
SRR3128743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861803
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135910.72999130412831No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC67570.36292776410823274No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC47590.2556124359021873TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG39490.2121062217646013No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG38370.20609054771100915No Hit
AAAGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT18930.1016756337808028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2650.085.125984
AGGGATG50250.082.2068255
AGGGTAC12800.079.304695
AGGGAAT20100.078.559425
AAGGGTA14650.078.2742544
CGTTTTT48600.078.129941
AAGGGAT38850.075.7268754
GAGGGAT30300.075.225634
TATAGGG14750.073.920132
CGTAGGG4350.073.4659962
GATGACC34700.073.269448
GAATAGG9800.072.978971
TAGGGCA8250.072.9159854
ATGGCAC37850.072.883179
TAGGGTA7750.072.7689744
CTACGAC13150.072.547985
GGGATGA40750.072.540096
GGATGGC42150.071.580137
CGGGATG10650.071.4859165
GGATGAC37150.071.346947