FastQCFastQC Report
Mon 27 Feb 2023
SRR3128742.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128742.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055254
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148020.7202029530169993No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC27820.13536039827680668Illumina Single End Adapter 1 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24990.12159081067352258No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG24380.11862280769189598Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62500.082.467461
ATAGCGG4350.073.783581
CGTAGGG6300.072.631872
TAAGGGA20100.071.8160253
AGGGATG43250.070.949695
AAGGGAT42000.070.872234
TAGAGGG38500.070.2085342
GAGGGAT29800.069.810114
TATAGGG18150.069.395392
TAGGGCA9550.069.154534
AGTAAGG12500.069.100491
ATAGGGA21800.068.812393
AGGGATT22950.068.798675
ATAAGGG27100.068.5840152
AGGGAAT23850.068.3698355
GAATAGG14450.067.941561
GGTAAGG13700.067.1821751
TAGGGTA7450.067.119024
AAGGGTA14250.066.539154
AGGGTAT10300.065.697485
Produced by FastQC (version 0.11.5)