FastQCFastQC Report
Mon 27 Feb 2023
SRR3128741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089076
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146330.7004532147226812No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC27490.13158927679031304Illumina Single End Adapter 1 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25380.12148911767690596No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG25050.11990947193879016Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62800.081.368531
AAGGGAT43650.071.923174
AGGGATG43150.071.449585
CGTAGGG7450.070.683022
AGATAGG13500.070.405061
TAGGGCA8900.070.232524
ATAGCGG5250.069.907141
AGGGAAT23350.069.6411365
ACGGGTA2700.069.626234
AGTAAGG13100.068.2446441
ATAGGGA19800.068.1229253
AAGGGTA14600.067.5994344
TAGGGTA7600.067.40464
AGTAGGG35700.067.03522
TAGAGGG41850.066.958262
GACCGAT1900.066.786218
GGGCGAT11050.066.775016
GAGGGAT28300.066.593924
AGGGTAC11000.066.224045
AGGGATT22750.066.106665
Produced by FastQC (version 0.11.5)