FastQCFastQC Report
Mon 27 Feb 2023
SRR3128740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200212
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149830.6809798328524705No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC31650.14384977447627775Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG26450.12021568830639956Illumina Single End Adapter 1 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25860.11753412852943262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62450.079.556111
ACGGGTA2100.076.090014
ATAGCGG4650.074.872241
AGGGATG42850.074.140245
TAGGGTA6800.072.568554
CGTAGGG6500.072.301082
AAGGGAT42350.071.3551944
AGATAGG15100.070.416351
GGTAAGG13500.070.3978961
GTAGGGC9500.070.24783
TAGACGG2750.070.144431
GAATAGG14250.069.994361
ATAGGGA20300.069.684873
TAGAGGG41400.069.585432
TAAGGGA20750.068.400123
AGGGAAT23500.067.993795
AGTAAGG15650.067.941661
TAGGGCA9450.067.635564
AGAGGGC31150.067.5908663
GAGGGAT32150.067.388844
Produced by FastQC (version 0.11.5)