FastQCFastQC Report
Mon 27 Feb 2023
SRR3128739.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128739.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180931
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160580.7362910610193537No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG29590.13567600258788565No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC27470.12595538327439063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82500.080.645011
AGTAGGG36800.070.371532
TAGGGCA10200.070.293614
AGGGATG34000.069.520925
ATAGGGC13450.068.739483
GAATAGG10050.068.0791851
ATAAGGG30450.067.851462
TAGGGTC6400.067.0708544
GTAGGGC10850.066.517233
TGTAGGG16600.066.493662
TAGAGGG40400.066.435982
AGGGAAT23250.066.294485
GTAGGGA15200.066.101073
AGGGTAC12100.066.02225
ATAGGGA19700.065.368553
AAGGGGC69850.065.2353744
ATAGAGG16350.065.223341
AAGGGAT35200.065.127754
AGAAGGG84050.064.989512
GAGGGAT27250.064.9138264
Produced by FastQC (version 0.11.5)