FastQCFastQC Report
Mon 27 Feb 2023
SRR3128737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315765
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160370.6925141367971275No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG32710.14124922002016613No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC32250.13926283539132858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83150.081.638561
AGTAGGG38950.071.068452
ATAGGGC14250.069.5883563
TAGGGCA11500.069.473654
AGGGATG37700.069.057265
TAGAGGG45150.068.1793062
ATAAGGG31500.067.884382
AGTAAGG15300.067.335471
GTAGGGC10850.067.1384053
AAGGGAT39150.066.623934
ATAGGGA21800.066.1836853
AGGGAAT21750.066.115475
AGGGCAT17350.066.089325
ATAGAGG17950.065.518931
ATGTAGG7800.065.1358951
GAGGGAT27900.065.0207754
AGGGTAC12000.065.008145
ATGCGGG11950.064.891092
AAGAGGG85300.064.8479842
TATAGGG19500.064.831592
Produced by FastQC (version 0.11.5)