FastQCFastQC Report
Mon 27 Feb 2023
SRR3128736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441642
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82490.5721947612514063No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC28680.19893982001079327No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27770.19262757328102262No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG24420.1693901814736252No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG21420.14858057686998574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG3950.088.402582
AGGGATG37600.081.735165
TAGGGTA3700.080.346914
CGTTTTT45000.078.9747851
ACGGGTA2300.077.9625554
AAGGGAT34200.074.369184
AGGGTAC7350.073.524065
ATAGAGG8500.072.182251
GTAGGGC6450.071.69633
TAGAGGG26050.071.551662
GAATAGG10800.071.231131
AAGGGTA12750.070.689274
TAAGAGG10700.070.573591
TAAGGGA15800.070.184483
AAGAGGG54700.070.048532
GGATGGC27850.069.5177
ATAGCGG3400.069.4060061
GATGACC22500.069.129678
AGTAAGG9900.069.125581
AGAGGGA39250.068.8882753
Produced by FastQC (version 0.11.5)