FastQCFastQC Report
Mon 27 Feb 2023
SRR3128734.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128734.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546311
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82880.5359853224868736No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29780.19258739024685204No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC28970.18734911670420762No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG27450.17751927005628235No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG24290.15708353623559557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG5300.082.466172
CGTTTTT45600.079.8510061
AGGGATG36700.078.117175
ATAGCGG1950.077.200421
AGGGTAC7250.077.142145
GACCGAT1350.076.5899358
GGTAAGG9200.076.1908341
AAGGGAT31500.075.791744
TAAGAGG12750.075.270411
TAGGGTA5200.074.110414
AGGGTAT11100.072.4028855
TAGAGGG28700.072.214672
GAGGGTA8150.072.0810554
ATAGGGC9850.071.0916753
AAGGGTA13300.071.0253144
CGGGTAT2250.071.019755
ACGGGTA2250.071.0174564
AGGGAAT18250.070.0468755
CGAAGGG15300.070.0345152
ATAGAGG10850.068.9400561
Produced by FastQC (version 0.11.5)