FastQCFastQC Report
Mon 27 Feb 2023
SRR3128733.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128733.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100854
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169840.8084331419508447No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG22570.10743250125901181No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC21940.10443372076308016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88500.084.623861
GAATAGG13450.072.5827941
ATAGGGC11700.071.73083
AGGGATG31050.069.16515
AGGGTAC11100.069.007625
AGTAAGG15450.068.986731
TAGAGGG43000.068.859322
TAAGGGA17650.068.653073
ATAGCGG2900.068.302551
TAGGGAT13300.067.001074
TATAGGG16350.066.902412
ATAGAGG16950.066.777041
GGTAAGG15250.066.4896851
AGTAGGG37450.066.348382
TAGGGCA10100.066.288554
AAGAGGG81550.066.02582
AGAGGGC31150.065.387873
GTAAGGG37300.064.592732
GAGGGAT27550.064.519554
GTACGGG4750.064.519552
Produced by FastQC (version 0.11.5)