FastQCFastQC Report
Mon 27 Feb 2023
SRR3128732.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128732.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127837
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168240.7906620666902586No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC23720.11147470412442306No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG23610.1109577472334582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86600.083.56211
ATAGCGG4050.073.1907961
GAATAGG13000.072.38651
ATAGGGC11450.068.957993
TAAGGGA17000.068.285553
GTACGGG4700.068.0293052
AGGGATG32350.067.119475
TAGAGGG40950.066.8273242
GGTAAGG14300.066.7931
AAGAGGG79000.065.947392
CGTAGGG5500.065.8283542
AAGGGGC68300.065.233164
AATAGGG31650.065.2192462
AGTAGGG37600.065.153072
ATAAGGG30450.064.855522
AAGGGAT34150.064.5450444
ATAGAGG17600.064.42811
AGAGGGC30550.064.151333
TATAGGG18750.063.696772
TAGGGCA10050.063.131854
Produced by FastQC (version 0.11.5)