FastQCFastQC Report
Mon 27 Feb 2023
SRR3128731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241499
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173360.7734110075445049No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG25310.11291550877336996No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC23800.10617894542892949No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92050.084.081061
GAATAGG12900.074.0393141
ATAGCGG3950.073.850031
AGGGATG32100.072.174575
TAGAGGG41500.070.549172
GGTAAGG13650.069.9712141
ATAGGGC12650.069.4709243
GAGGGAT28900.066.833784
ATAGAGG17850.066.686571
TAAGGGA17650.066.565213
AGTAGGG38350.066.418022
AAGAGGG88800.066.364612
AAGGGAT35750.065.858784
GCATAGG6750.064.823911
AAGGGAC19900.064.706734
AAGGGGC72550.064.516964
AGTAAGG15300.063.9628751
AGAGGGC33700.063.868623
AGAGGGA53000.063.4876373
TAGGGAT13200.063.3720934
Produced by FastQC (version 0.11.5)