FastQCFastQC Report
Mon 27 Feb 2023
SRR3128730.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128730.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300515
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142760.6205567014342441No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC38420.1670060834204515No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG32220.14005559624692732No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG27870.12114678669776116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65200.075.231051
ATAGGGC11750.073.433123
TAAGGGA18700.072.6155553
GTAGGGC10150.072.466513
AGGGATG32100.072.3192755
GAGGGAT31550.070.836614
ATAGGGA18750.070.158733
CGTAGGG5650.070.098652
GAATAGG13550.069.6148761
TAGGGCA10350.068.788434
GGATAGG13700.068.164151
TATAGGG19700.067.732792
AAGGGAT38250.067.4271244
ACGGGTA2450.067.356694
AGTAAGG14150.067.329641
AGTAGGG38050.067.285882
GGTAAGG13900.067.183371
TGTAGGG16300.067.108752
TAGAGGG42250.067.0697252
AGGGTAC9050.066.9843445
Produced by FastQC (version 0.11.5)