FastQCFastQC Report
Mon 27 Feb 2023
SRR3128729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345845
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140210.5976950736301844No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC40850.1741376774680339No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG34580.14740956883340545No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG30240.12890877274500234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64600.076.916971
GTAGGGC9900.073.581493
TAGGGCA11700.071.901714
ATAGGGC13700.071.3534243
AGGGATG33150.071.169225
AGGGTAC10150.070.380065
TAAGGGA18400.070.240393
AAGGGTA15000.070.1824954
ATAGCGG4300.070.032921
CGTAGGG6750.069.661132
AAGGGAT39300.069.359754
ATGCGGG12400.067.877472
AGTAGGG38350.067.681192
AGTAAGG13950.067.122731
ATAGGGA20100.067.105473
ATAGAGG15250.066.954631
GAATAGG12800.066.9040151
TATAGGG18000.066.352232
GGTAAGG14900.066.316831
CGAAGGG25150.065.998032
Produced by FastQC (version 0.11.5)