FastQCFastQC Report
Mon 27 Feb 2023
SRR3128728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431716
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143440.5898715146012116No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC42780.17592514915393082No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG35550.1461930587288976No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG32800.1348841723293345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66050.076.980721
CGTAGGG7550.075.942452
TAGGGCA10250.073.3614354
ATAGGGC13800.073.220233
AGGGTAC10200.070.9550555
TAAGGGA20600.070.952043
ATAGAGG18000.070.5537951
TAGAGGG48100.069.567542
ATAGGGA18850.068.5635153
AGGGATG33250.068.550865
AAGGGAT35750.068.490984
TATAGGG19750.067.342792
AGTAAGG13250.067.092661
GCATAGG6600.066.990461
GAGGGTA13350.066.887364
GAATAGG14100.066.717531
TAGACGG3750.066.477341
ATAAGGG34800.066.4442752
GATAGGG34650.066.325012
AGTAGGG38850.066.292022
Produced by FastQC (version 0.11.5)