FastQCFastQC Report
Mon 27 Feb 2023
SRR3128727.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128727.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125744
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137220.6455151702180507No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC25790.12132222882905938No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG22090.10391655815563869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56950.080.288251
ATAGGGC12250.073.5262453
AGGGATG32700.072.8602755
AGTAGGG36800.071.7604752
TAAACGG3700.071.418091
TAGGGTA6550.071.275284
CGTAGGG7100.071.067422
TAAGGGA20800.070.735323
AGGGAAT20850.069.643885
TATAGGG16000.069.261672
GAATAGG12700.068.365361
TAGGGCA11000.068.1631244
GTAGGGT7850.067.881883
AAGAGGG82150.067.67862
GAGGGTA11500.067.249814
AGGGTAC10000.067.199595
ATAGGGA18900.066.8679663
GGATAGG12150.066.7996441
ATAAGGG30100.066.740312
GGGCGAT9650.066.715066
Produced by FastQC (version 0.11.5)