FastQCFastQC Report
Mon 27 Feb 2023
SRR3128726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158355
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134190.621723488490077No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC29630.1372804751766971No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG22220.10294877348721596No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG21690.10049319968216536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55300.078.2139361
ATAGGGC12650.075.046483
AGGGAAT20450.074.68955
TAGGGTA5700.073.381184
TAAGGGA19550.072.598843
ATAGGGA20000.070.730383
AGTAAGG15450.070.3683241
GAATAGG13850.070.002121
AGGGATG33100.069.714485
TATAGGG15850.069.428522
AGATAGG13050.068.883721
TAGAGGG41950.068.607362
ATAGCGG3850.068.45751
ATAGAGG16300.067.853771
AAGGGGC65000.066.301834
AGTAGGG36400.066.019282
CGAAGGG26850.065.855932
GTAAGGG36150.065.82542
CGTAGGG5800.065.676252
AATAGGG35700.065.6012952
Produced by FastQC (version 0.11.5)