FastQCFastQC Report
Mon 27 Feb 2023
SRR3128725.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128725.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257188
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142770.6325126662023722No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC29250.1295860158746192No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG24650.10920667662596116No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG23700.10499790004199916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61950.078.54031
ATAGGGC10850.073.630023
CGTAGGG6850.073.405552
TATAGGG16600.072.188462
AGGGATG35600.071.415535
GAATAGG13150.070.494261
TAAGGGA18650.069.796993
TAGAGGG45400.069.5583342
TAGGGTA5350.069.391944
AGGGAAT19250.069.331875
GGTAAGG12100.069.222611
ATAGAGG17750.069.191931
GTACGGG4850.068.794282
AGATAGG12450.068.788431
AGTAGGG36600.068.435562
ATAGGGA20400.068.416693
CGAAGGG27850.068.0010452
AGTAAGG15600.066.964051
ATAAGGG31400.066.299442
AAGGGAT38150.065.039194
Produced by FastQC (version 0.11.5)