FastQCFastQC Report
Mon 27 Feb 2023
SRR3128724.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128724.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207666
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143260.6489206247684206No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC23600.10690022856718363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65850.076.918391
ATAGGGC12900.074.5545353
GAATAGG14200.074.394591
GTACGGG4300.072.3617552
TAGGGCA10850.071.694834
GGTAAGG14850.069.550351
ATAACGG2200.068.597611
AAGAGGG86000.068.195482
AGTAGGG36800.067.8987352
ATAGGGA18150.067.0157243
TAGAGGG42050.066.933262
ATAAGGG29400.066.708262
ATAGAGG14700.066.410191
AGGGATG33700.066.236665
AGAGGGC34900.066.191813
ACGGGTA2000.066.002694
AGATAGG13450.065.9200061
AAGGGTA15650.065.6713264
TAACGGG8000.065.413382
AGTAAGG13250.064.7794341
Produced by FastQC (version 0.11.5)