FastQCFastQC Report
Mon 27 Feb 2023
SRR3128723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233862
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136040.6089901703865324No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC25750.11527122087219353No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG23480.10510944722637298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62800.077.566971
GAATAGG13150.071.939341
AGTAGGG38500.069.9921652
TAGGGCA11150.069.5476764
ATAGGGC13200.069.427933
TAGAGGG41900.069.363342
ATAGAGG16700.069.047151
ATAGGGA19150.068.2259453
AGATAGG14200.067.282771
AGGGATG33500.067.199235
GTAGGGC11600.066.84973
TATAGGG16700.066.740192
CGTAGGG5850.066.723342
AGAGGGC34000.065.934553
ATAACGG2450.065.314411
AGGGAAT21200.064.953925
AGTACGG2700.064.496191
ATAAGGG29500.064.4043052
CGAAGGG23000.063.9988332
AATAGGG33850.063.9079252
Produced by FastQC (version 0.11.5)