FastQCFastQC Report
Mon 27 Feb 2023
SRR3128722.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128722.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2368984
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139860.590379673311428No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC26670.11257990767350053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67350.078.72831
ATAGGGC13350.075.329223
TAGGGCA11700.074.706444
GAATAGG14200.073.885821
TAACGGG9300.070.741792
AGATAGG14350.070.162721
AGGGATG35500.069.628715
GGTAAGG13400.068.465911
ATAGGGA18700.068.35313
ATAAGGG32050.068.1798252
AAGAGGG89200.067.696972
AGTAGGG40750.067.692642
ATAGCGG5200.066.9534451
TATAGGG17600.066.484082
TAGAGGG47650.066.174492
TAAGGGA18650.066.016643
AATAGGG34000.065.928082
AGAAGGG87000.064.601552
AGGGCAT20400.064.269515
ATAGAGG17100.064.106841
Produced by FastQC (version 0.11.5)