FastQCFastQC Report
Mon 27 Feb 2023
SRR3128721.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128721.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738943
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138370.7957132579963805No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC19260.11075693682886673Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55450.080.374621
ATAGGGC9000.075.423753
CGTAGGG5150.073.2269442
TAGGGTA6000.072.2811054
ATAGAGG12750.070.649941
TAGAGGG32850.070.602152
GTACGGG3750.070.395512
AGGGATG28600.070.3258745
ATAGGGA15500.069.6453253
AGGGAAT15600.068.983995
GAGGGAT20100.068.950814
AGATAGG11650.068.819521
GAATAGG12200.068.809561
AGTAGGG29750.068.7687152
ATAGCGG4500.068.122331
AGGGATA17100.067.879445
TAAGGGA14750.067.753553
ACGGGTA2650.067.596764
GATAGGG28650.067.460162
AGGGTAC9650.067.203085
Produced by FastQC (version 0.11.5)