FastQCFastQC Report
Mon 27 Feb 2023
SRR3128720.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128720.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791546
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133930.7475666268128197No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC21110.11783119160769526Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG17980.10036024751806541TruSeq Adapter, Index 22 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58600.080.227461
CGTAGGG4550.074.427532
ACGGGTA2050.073.361654
AGGGATG30650.071.300975
AGGGTAC10400.070.495965
AAGGGTA12250.070.208224
GAATAGG12650.069.939491
ATAGAGG13500.069.3704451
ATAGGGC11150.069.126083
GTACGGG4050.068.518762
TAGAGGG33400.068.2979662
TAGGGTA6000.068.1460954
GAGGGAT21900.068.028064
AGTAGGG31400.067.7050862
AGATAGG13000.067.3325041
GGTAAGG10450.066.199731
AAGGGAT31450.065.75144
ATAGCGG4300.065.665611
AAGGGGC58350.065.482114
ATAGGGA16300.065.450233
Produced by FastQC (version 0.11.5)