FastQCFastQC Report
Mon 27 Feb 2023
SRR3128719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1949151
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137740.7066666461449114No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC20720.10630269281343518Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60650.081.616091
ATAGGGC12200.072.808153
ATAGAGG15000.072.77591
CGTAGGG5950.072.668952
AGGGTAC10850.071.47145
GAATAGG12900.070.39771
GTAGGGT7300.069.5310753
TAGGGTA6300.069.377794
AGGGATG29200.069.048225
TAGAGGG37650.067.90662
TAGGGCA8800.067.8264544
AGTAGGG31500.067.7365952
ACGGGTA2850.067.6109544
GTACGGG5300.067.393162
GAGGGAT22050.067.3529364
GGGTACC14000.067.138076
AAGGGGC63650.066.306494
AATAGGG30500.066.105192
ATAGCGG4600.065.4655761
AAGAGGG74100.065.264232
Produced by FastQC (version 0.11.5)