FastQCFastQC Report
Mon 27 Feb 2023
SRR3128718.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128718.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903585
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134770.7079799431073475No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC25910.13611159995482208No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG25160.13217166556786275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68400.080.829671
ATAGGGC10850.076.060433
GAATAGG12000.075.4778061
TAGGGCA10750.073.697254
CGTAGGG6050.073.2694552
AGGGTAC10800.070.926075
TATAGGG16150.070.079212
ACGGGAT5500.069.450094
AGGGCAT16500.069.209285
AGGGATG29650.067.8361365
ATAGCGG3900.067.73651
GTAGGGC9100.066.84963
AAGGGAT30750.066.5572054
AAGGGTA17150.066.542894
AAGAGGG68300.066.282822
TAGAGGG35650.065.874952
TAGGGTA6100.065.711234
AGTAGGG32000.065.5783542
GAGGGAT23500.065.217774
ATAGGGA15600.064.992663
Produced by FastQC (version 0.11.5)