FastQCFastQC Report
Mon 27 Feb 2023
SRR3128717.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128717.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944745
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130740.6722732286238041No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC29120.149736854960419No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG26110.1342592473563372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61950.080.420381
TAGGGCA10600.072.713284
ATAGGGC11100.071.978323
AGGGTAC11300.071.9520955
GAATAGG10400.071.019521
TAGGGTA5650.070.704384
AAGGGAT29150.069.166294
AGGGCAT16750.069.023355
ATAGCGG3900.068.757761
AAGGGTA18550.067.899514
TAGAGGG35750.067.869212
GTAGGGC9700.067.83163
CGTAGGG7100.067.552432
AGGGATG31450.066.947315
AGTAGGG29600.066.878972
AGGGTAT10850.066.706285
AGAGGGC28500.066.291373
TGTAGGG16200.065.888562
ATAGAGG14500.065.862691
ACGGGAT5500.065.7966464
Produced by FastQC (version 0.11.5)