FastQCFastQC Report
Mon 27 Feb 2023
SRR3128716.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128716.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047177
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135360.6612032081251401No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC30730.15010915030796065No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG27570.13467325981094944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65500.081.738411
GAATAGG12800.075.347541
TAGGGCA9250.071.6402054
TAGAGGG41600.069.932142
ATAGGGC10850.069.3058243
AGTAGGG33200.069.081412
AGGGTAC11050.068.049755
CGTAGGG6700.068.041892
AAGGGTA17850.067.929894
AGTTAGG6500.066.588561
GGTAAGG14350.066.553261
AAGGGAT30200.066.450834
AGAGGGC31500.066.244823
AGGGATG31800.066.061625
GATAGGG31550.065.990872
TAAGGGA18250.065.925973
AGGGTAT12250.065.220135
AAGAGGG72000.065.209732
TGTAGGG13500.065.1009452
AAGGGGC61250.064.531144
Produced by FastQC (version 0.11.5)