FastQCFastQC Report
Mon 27 Feb 2023
SRR3128715.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128715.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201047
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC11660.579963889040871No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG11340.5640472128407785No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG8320.4138335812024054No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.23327878555760595No Hit
AGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT3530.17558083433227056No Hit
GGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT2760.13728133222579794No Hit
AAGAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2640.13131257865076326No Hit
AGAGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT2590.1288255979944988No Hit
ACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG2480.12335424055071699No Hit
ACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT2140.1064427720881187No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT2110.10495058369436001No Hit
GGAAAGGTGGGGAAAAGATAGCGAAATCAGATTGTTGCTGTGTCTGTGTA2090.10395579143185425No Hit
AAAGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT2040.1014688107755898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG450.094.270211
AATGCGG156.8156276E-494.270211
CCGAAGG156.8156276E-494.270211
GCACGGG301.29657565E-894.246692
AGCGTAC156.897125E-493.98889
CGGGGAT301.3214958E-893.98885
GATAGCT156.897125E-493.98888
AGGGTAT1100.085.4443745
TAGCGGG900.083.774842
CGAAGGG1300.083.3720862
GATTAGG800.082.4864351
CGTTTTT5850.082.184291
AAGGGTA1150.081.953654
AGTAAGG1500.081.700851
GTAGGGT750.081.680473
GGGCGAT1450.081.024836
TAGAGGG3150.080.782882
AGGGCGT353.851528E-880.561845
GAGTAGG1000.080.129681
AGAGGGC2250.079.58613
Produced by FastQC (version 0.11.5)