FastQCFastQC Report
Mon 27 Feb 2023
SRR3128714.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128714.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206972
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC12830.6198906132230447No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG11660.5633612276056665No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG8860.42807722783758195No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.22031965676516632No Hit
AGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT4220.2038923139361846No Hit
GGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT2870.13866609976228667No Hit
ACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG2810.13576715691011346No Hit
AAGAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2650.12803664263765147No Hit
AGAGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT2590.12513769978547823No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT2440.11789034265504512No Hit
AGGCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT2400.11595771408692962No Hit
AAAGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT2180.1053282569622944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG254.8514994E-794.179131
ACCGAGG156.8421604E-494.179131
CATACGG409.094947E-1294.179131
GGTTAGG254.8514994E-794.179131
CTAGCGG156.8421604E-494.179131
TTAGGGT156.8949955E-493.99713
GGAATCG201.8346029E-593.99717
GGGTACG201.8346029E-593.99716
TTCACGA156.8949955E-493.99719
ACGGGTA301.3207682E-893.99714
GCACCGA254.907761E-793.99718
GCGATAG254.907761E-793.99718
TCGAGCG156.8949955E-493.997194
CGATAGG156.8949955E-493.99719
CGAGGGT550.085.451913
AGTAAGG1550.085.0650251
AGGGATG1550.084.900615
GGGTAGT500.084.597396
CGTTTTT6350.083.797181
TAGCGGG900.083.63382