FastQCFastQC Report
Mon 27 Feb 2023
SRR3128713.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128713.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212981
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC12810.6014621022532527No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG12650.5939496950432198No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG9070.42585958371873545No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.22631126720223868No Hit
AGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT4060.1906273329545828No Hit
ACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG2860.13428427887933664No Hit
AGAGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT2720.12771092257055794No Hit
AAGAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2570.12066804081115216No Hit
GGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT2520.11832041355801691No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT2460.11550326085425461No Hit
GAGAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2270.10658227729234064No Hit
ACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT2260.10611275184171359No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG353.5652192E-1094.0427551
GAGCGAC201.8345798E-593.998694
GTACAGG800.088.165091
GATTAGG1100.085.493421
CGTAGGG550.085.43322
AAGGGTA1500.084.5788654
GGGTATC1400.083.90766
TAGCGGG900.083.534682
CGTTTTT5700.083.318591
TAACGGG401.033186E-982.2294542
GTACGGG750.081.446322
AATGCGG353.83734E-880.608081
ACGTAGG353.83734E-880.608081
AGTACGG353.83734E-880.608081
TAAGCGG650.079.5746461
TAGGGTC650.079.518594
AGGGTAG1250.078.940285
AACGAGG301.448132E-678.3689651
GTAAGAG301.448132E-678.3689651
AGTAAGG1500.078.3689651
Produced by FastQC (version 0.11.5)