FastQCFastQC Report
Mon 27 Feb 2023
SRR3128712.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128712.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10828370
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3829613.5366449428676705No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT219640.20283754618654515No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG199390.18413667061616845TruSeq Adapter, Index 23 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT186800.17250980526154905No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT183600.16955460517141543No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT164180.15162023462441715No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA140060.12934541394503513No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC109150.10080002807440086TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1154200.088.830861
ATAGGGC48350.071.296333
AGTAGGG156850.068.7254942
AAGGGGC397950.068.695184
GGATAGG58750.068.4976961
GGTAAGG63200.068.3719941
GTTTTTT1514950.068.2949142
AGATAGG54900.068.15131
ATAGAGG67200.067.387621
ATAGGGA57350.066.923223
TAGTAGG36300.066.464431
GATAGGG146400.065.9109342
AATAGGG132400.065.624352
TAGAGGG191250.065.1792142
AGGGACT155650.064.975325
AGGGGCT482350.064.956625
GAATAGG61300.064.9564361
GTATAGG34250.064.66411
TATAGGG71900.064.449982
AGTAAGG61200.064.36961
Produced by FastQC (version 0.11.5)