FastQCFastQC Report
Mon 27 Feb 2023
SRR3128711.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128711.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7282908
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2372383.2574625410618943No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT209130.2871517805799551No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT192190.26389184100636726No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG187990.258124913839362TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT167290.22970220137340744No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT110570.1518212230608982No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC107970.14825122052894255TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTC87560.12022670065309077No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA85010.11672535201598044No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC84370.11584658216196057No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC75780.1040518430275379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT746600.088.94041
ATAGGGC29850.072.112583
GGTAAGG41050.070.455411
GTTTTTT971200.068.8344652
AAGGGGC260200.068.797984
AGTAGGG102750.068.795322
GGATAGG39050.068.403721
GAATAGG36250.067.84941
TAGTAGG24200.066.6547551
ATAGAGG44450.066.5474241
ATAAGGG80050.065.9932
AATAGGG85500.065.634342
AGGGACT92550.065.4549565
TATAGGG49150.065.407182
TAATAGG16600.065.158651
AGGGGCT315750.064.79055
AGTAAGG41950.064.57181
AGATAGG33800.064.4193951
GTATAGG21800.064.285371
ATGTAGG17700.064.031871
Produced by FastQC (version 0.11.5)