FastQCFastQC Report
Mon 27 Feb 2023
SRR3128710.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128710.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10889411
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3641553.3441202650905546No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT230790.21193983770104738No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG202440.18590537174141009TruSeq Adapter, Index 23 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT190340.17479365963870774No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT187480.1721672549598872No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT165800.15225800550645027No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA140570.12908870828734448No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC117350.10776524092992726TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1119000.088.79321
ATAGGGC45500.071.580153
GGATAGG55300.069.514351
GGTAAGG59300.069.348051
TAGTAGG37250.069.220031
AGTAGGG163900.069.16662
AAGGGGC392850.068.3691564
GTTTTTT1481250.067.787472
ATAGGGA56300.067.699153
ATAGAGG74800.066.740791
TATAGGG73900.066.552662
AGATAGG55800.066.4461061
AATAGGG131450.066.138532
AGGGACT154250.066.054865
GAATAGG59900.065.904141
GATAGGG145150.065.27342
TAGGGCA32050.064.6667944
TAGAGGG201450.064.629962
AGGGGCT489700.064.588575
ATAAGGG134400.063.6722682
Produced by FastQC (version 0.11.5)