FastQCFastQC Report
Mon 27 Feb 2023
SRR3128709.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128709.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12795033
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4428113.460803891635137No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG231560.1809764773564867TruSeq Adapter, Index 23 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT221840.17337977948161604No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT219430.17149623607848452No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT200960.15706094700967166No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT179210.14006216318472958No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA166780.1303474559229351No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1345700.088.6376341
ATAGGGC53550.072.690163
AAGGGGC471200.068.712324
ATAGAGG83700.068.642931
AGTAGGG194550.067.858822
GTTTTTT1782300.067.707172
GGTAAGG72650.067.424961
GGATAGG67200.066.731251
TATAGGG93100.066.30642
ATAGGGA72750.066.300853
AGATAGG66550.065.4032441
AGGGGCT576800.065.326315
AATAGGG157850.065.19912
TAGTAGG46850.064.983081
TAGAGGG233250.064.8442
GATAGGG173050.064.7158362
GTATAGG42850.064.680011
AGGGACT175600.064.146375
ATAAGGG152950.063.968032
AGTAAGG75500.063.9449121
Produced by FastQC (version 0.11.5)