FastQCFastQC Report
Mon 27 Feb 2023
SRR3128708.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128708.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9644774
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3775673.9147314390155743No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT242170.25108934641703373TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT241250.2501354619610579No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT240740.24960667818654955TruSeq Adapter, Index 23 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT174990.18143504451218867No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG127680.13238257319456112Illumina PCR Primer Index 7 (95% over 22bp)
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA124330.12890918957769254No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTC113360.11753515427111096No Hit
CGTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC112690.11684047754773724TruSeq Adapter, Index 23 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1060650.089.332461
ATAGGGC45150.073.529313
GGTAAGG52050.069.523461
AGTAGGG138000.069.5097352
GTTTTTT1395450.068.382432
AAGGGGC331500.068.142074
AGATAGG47000.068.071111
AGTAAGG57050.067.8902051
ATAGAGG59800.067.84111
TAGTAGG31300.067.591631
GGATAGG53150.066.488861
GAATAGG54850.066.060311
AATAGGG115900.065.780492
TATAGGG64850.065.6435552
TAGAGGG166100.065.234992
GATAGGG130100.065.2245562
AGGGACT138300.064.801225
ATAGGGA55450.064.7965853
AGGGGCT412350.064.7802665
GTATAGG29300.064.16471
Produced by FastQC (version 0.11.5)