FastQCFastQC Report
Mon 27 Feb 2023
SRR3128707.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128707.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6603501
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2268603.435450377004562No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT245030.3710607448988045TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT239310.3623986730675137No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT228080.34539254253160556TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG120310.18219123461933298Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTC109840.1663360087323376No Hit
CGTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC107580.16291358175004442TruSeq Adapter, Index 23 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT104750.15862797628106667No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC80520.12193531885586145TruSeq Adapter, Index 23 (96% over 25bp)
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA77780.11778600472688655No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT658300.089.39911
GTATAGG18950.070.232491
GGTAAGG35100.070.207751
GGATAGG34400.069.859151
ATAGGGC29450.069.7408453
AGTAGGG93600.069.59562
AGATAGG30900.069.096851
TATAGGG44100.068.9540942
GTTTTTT867900.068.0056842
AAGGGGC224950.067.816784
GAATAGG35950.066.45471
AATAGGG74150.066.1100542
AGTAAGG35400.066.025851
ATAGGGA31650.065.487133
AGGGACT86600.065.122555
TAGTAGG23400.065.116351
ATAGAGG40050.064.583421
GATAGGG88000.063.7699472
AGGGGCT287300.063.5348055
TAATAGG14900.063.441091
Produced by FastQC (version 0.11.5)