FastQCFastQC Report
Mon 27 Feb 2023
SRR3128706.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128706.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9601059
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3555773.703518538944506No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT249770.2601483857145342TruSeq Adapter, Index 23 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT244290.2544406820122655TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT243440.2535553630073516No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT178160.18556286342996123No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG128120.1334436128347925Illumina PCR Primer Index 7 (95% over 22bp)
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA127030.13230832140496168No Hit
CGTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC115720.12052837088075388TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTC114690.11945557255715229No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT96180.10017644928543819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1004050.089.0032651
ATAGGGC43650.071.920623
AGTAGGG135000.069.101322
GGTAAGG55000.068.359281
AGATAGG46400.068.1498261
GTTTTTT1326650.067.8932952
AAGGGGC336650.067.426374
GGATAGG51250.066.474971
ATAGAGG58700.065.9743651
TATAGGG62250.065.8651352
AGGGACT134400.065.52875
AGTAAGG51250.065.005911
GATAGGG126250.064.989392
AATAGGG118700.064.963822
GTATAGG29200.064.94351
TAGTAGG31950.064.8030241
AGGGGCT416750.064.7062455
GAATAGG55650.064.685911
ATAGGGA53450.064.537123
TAGGGCA29800.064.5012744
Produced by FastQC (version 0.11.5)