FastQCFastQC Report
Mon 27 Feb 2023
SRR3128705.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128705.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11588477
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4413683.808679949919217No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT260960.22518921166258518No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT256410.22126289761803902TruSeq Adapter, Index 23 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT242240.20903523387930958TruSeq Adapter, Index 23 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT211850.18281090776639586No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG154510.13333072154347805Illumina PCR Primer Index 7 (95% over 22bp)
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA152810.131863747065296No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTC127830.1103078514976558No Hit
CGTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC126010.10873732587983735TruSeq Adapter, Index 23 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT116330.10038420061583588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1221700.088.984391
ATAGGGC52300.073.6239243
AAGGGGC407600.068.827554
AGTAGGG165450.068.659792
GTTTTTT1615350.067.779852
TAGTAGG38400.067.1462551
TATAGGG82150.066.793722
GGATAGG59300.066.411481
GGTAAGG63150.066.237021
GTATAGG37700.065.8969041
ATAGGGA62650.065.7384953
AGATAGG56950.065.351451
AGGGACT166650.065.338625
AATAGGG138050.065.155482
ATAAGGG136600.065.0898362
AGGGGCT505950.064.9168855
ATAGAGG73100.064.816341
TAGGGCA36800.064.390714
GATAGGG151850.064.157482
TAGAGGG212350.063.990862
Produced by FastQC (version 0.11.5)