FastQCFastQC Report
Mon 27 Feb 2023
SRR3128704.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128704.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121448
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189320.8924093355104628No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG35330.1665371953495914No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34190.1611635071894291No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG30800.14518385555526225No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC29670.13985730501053997Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22930.10808655220396635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62650.082.6991041
CGTAGGG6150.074.146492
TAGGGTA6700.073.650454
AGTAGGG33900.073.497092
AGGGATG28500.070.411565
CTACGAC6700.070.143295
GTAGGGA16350.068.984963
GAATAGG12500.068.516621
AAGGGAT34550.068.4196554
ACGGGAT7550.067.848534
TATAGGG16350.067.5684742
GTAGGGC7750.067.310413
ATAGGGC11750.067.194283
TAAGGGA18200.067.137153
TAGCGGG10800.066.598082
AGGGCAT16500.066.364055
GGTAAGG11550.066.003591
AGTAAGG11850.065.523961
TAGAGGG34700.065.164282
GTAAGGG31750.064.852152
Produced by FastQC (version 0.11.5)