FastQCFastQC Report
Mon 27 Feb 2023
SRR3128703.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128703.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001325
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175700.8779183790738635No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG40410.20191623049729554No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG34620.17298539717437197No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC34130.17053701922476358Illumina Single End Adapter 1 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31800.1588947322398911No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCAAATGC24940.12461744094537368No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20340.10163266835721335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57350.082.971741
ATAGCGG4050.081.269221
CGTAGGG4850.074.613082
CTACGAC7650.074.334415
TAGGGTA6950.072.35444
ACGGGAT8150.072.0806054
GACCGAT1500.072.059588
AGGGATG27000.071.713265
TAGCGGG10300.071.635522
AGTAGGG31800.071.085982
AGGGCAT17300.070.902315
GAATAGG11150.070.4246441
ATAGGGC11100.068.589573
TATAGGG15900.068.5735862
ACGGGTA1650.068.358634
TCCGTAC13750.068.358622
AGTAAGG12250.068.3230061
TAAGGGA16200.067.88393
AAGGGAT33650.067.876154
CCGTACA12950.067.863753
Produced by FastQC (version 0.11.5)