FastQCFastQC Report
Mon 27 Feb 2023
SRR3128702.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128702.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240168
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215500.9619814228218597No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG44720.1996278850514783No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG39400.17587966616789455No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC38240.170701483103053Illumina Single End Adapter 1 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36530.16306812703332965No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23960.10695626399448613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65300.082.653831
ATAGCGG4500.078.426261
GACCGAT2150.074.32248
CTACGAC7550.073.453815
AGTAGGG35350.072.73042
GAATAGG12250.071.06381
TAAGGGA20050.070.5555953
TAGCGGG10700.070.2836462
AGGGATG29250.070.055125
ATAACGG2500.069.642521
AGGGCAT18050.069.520585
TAGGGCA9450.069.1293264
ATAGGGC11900.069.114693
TAGGGTA8100.069.046434
GACGGGT4400.068.360723
GGTAAGG13500.067.969431
GTAGGGC7400.067.321453
CGTAGGG5100.067.277632
AAGGGTA12750.066.718724
ATAAGGG29800.066.717872
Produced by FastQC (version 0.11.5)