FastQCFastQC Report
Mon 27 Feb 2023
SRR3128701.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128701.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521143
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162771.0700506132559529No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG54240.35657397102047605TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG53700.35302400891960845TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC48030.31574940686049896Illumina Single End Adapter 1 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTCTCTGC38190.2510612085780233No Hit
AGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT15620.10268594076953974No Hit
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT15550.10222576049720507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52700.082.960711
CGTAGGG2850.082.480172
AGGGTAC6200.075.040645
TAGGGCA8450.074.524864
TTACGCG200.002164000270.492737
CGAAGGG9700.070.278212
GAATAGG8050.070.1540451
GCAAGGG18700.070.1434252
GTAGGGC6450.069.9462663
ACGAGGG13100.069.623342
AAGGGAT20900.069.480874
ACGGGTA1700.069.110524
TAGGGTA5450.068.983714
ATAGGGC8850.068.501413
GTAGGGA12650.068.356583
TAAGGGA11700.068.2835543
AGGGCAT13800.068.108925
AGTAGGG21700.067.812382
GTAGAGG10250.067.034181
TAAGCGG3800.066.877111
Produced by FastQC (version 0.11.5)