FastQCFastQC Report
Mon 27 Feb 2023
SRR3128700.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128700.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463829
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150761.0299017166622604No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG59850.4088592315085984TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG57720.3943083515902472TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC54480.37217461875669905Illumina Single End Adapter 1 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTCTCTGC49100.3354216920145728No Hit
AGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT17480.11941285491679698No Hit
ACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG16850.11510907353249594No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT16770.11456256161067994No Hit
GGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT16340.11162506003091892TruSeq Adapter, Index 23 (95% over 22bp)
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATG16000.10930238436320089No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA15550.10622825480298585No Hit
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT15420.10534017293003486No Hit
GGAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT15160.10356400918413283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2900.085.900272
CGTTTTT47700.081.638631
ATAGGGC8300.073.051353
TATAGGG8900.072.351442
GAATAGG7550.072.2594761
AGTAGGG19950.070.9153442
TAGGGCA6450.070.682814
GTAGGGA8850.070.6358263
AGGGCAC9500.069.758525
AATAGGG16950.069.601832
GTATAGG3900.068.737551
AAGGGAC10750.068.642484
CCGTACA20900.068.14173
AGGGAAT17900.068.00625
AGGGTAC5750.067.84415
ATCCGTA22000.067.553551
GGTAAGG6900.067.4791951
CGAAGGG10050.067.34622
TCCGTAC22100.067.206532
AAGGGAT19900.067.075994
Produced by FastQC (version 0.11.5)